"Last Refrain"
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The "Last Refrain" is a Collectible in Mizuki & Caerula Arbor.
Item description
When obtained, randomly gain 2 Revelations[sic]
Cry, cry, for where are the Abyssal Hunters now?
Alas, alas, there is nothing in the ocean but the sound of the tide.
Effect
Applies two random Enlightenments when obtained.
Acquisition
SPOILER
The Last Refrain is obtained in Encounter: Paean, which will only appear when the player has the Caerula Animus.
Since the Caerula Animus is obtained from the namesake Encounter which will only appear when the player has the Bishop's Research which itself is obtained from Encounter: The Beginning which will only appear in the following runs after the player had completed a run with the Age of the Silence ending, it is impossible to obtain the Last Refrain before then.